紫外燈的應(yīng)用：薄層色譜法 Thin-layer Chromatography (TLC)

紫外燈波長(zhǎng)的選擇：根據(jù)TLC板類型選擇波長(zhǎng)，短波(254nm)可用于二氧化硅涂層，長(zhǎng)波(365nm)可用于纖維素涂層

WAVELENGTHSI LAMPS USED: Shortwave (254nm) or longwave (365nm) depending upon the type of TLC plate used (Silica coated =254nm, Cellulose coated = 365nm)

薄層色譜法(TLC)是一種微量、快速的層析方法。在生物、醫(yī)藥、化工各個(gè)領(lǐng)域都有廣泛的應(yīng)用。色譜法使化學(xué)家能夠根據(jù)其他已知標(biāo)準(zhǔn)測(cè)量給定溶劑中未知化合物的溶解度，從而將化合物混合物分離為其成分。薄層色譜 (TLC) 在TLC板上完成，該TLC板由固體支撐物（即玻璃、塑料或鋁）組成，并涂有一層薄薄的吸收材料（例如二氧化硅或纖維素）。TLC 板分為“泳道” 當(dāng)板的一端放入溶劑中時(shí)，已知和未知的樣品通過毛細(xì)作用沿著吸收材料沉積和遷移。相同的化合物將遷移相似的距離并表現(xiàn)出相同的濃度。

Chromatography enables the chemist to separate a mixture of compounds into its constituents by measuring solubilities of unknown compounds in a given solvent against other known standards. Thin-layer Chromatography (TLC) is done on a TLC plate consisting of a solid support, i.e., glass, plastic, or aluminum, coated with a thin layer of absorbent material, such as silica or cellulose.TLC plates are divided into “l(fā)anes" where known and unknown samples are deposited and migrate along the absorbent material by capillary action when one end of the plate is placed in solvent. Identical compounds will migrate similar distances and exhibit equal concentrations.

TLC薄層色譜法步驟

已知和未知樣品被“點(diǎn)樣”到TLC板的泳道上，并用適當(dāng)?shù)娜軇┻w移。通過將板從溶劑中取出并用吹風(fēng)機(jī)從泳道中蒸發(fā)溶劑來固定板。溶解的化合物將留在泳道的吸附材料中。然后通過向該板噴灑一種溶液來顯影該板，該溶液可誘導(dǎo)存在的化合物的熒光。在析浦紫外燈下照射，如果未知化合物與標(biāo)準(zhǔn)品遷移相同的距離并表現(xiàn)出相同程度的熒光，則認(rèn)為它們與已知標(biāo)準(zhǔn)品相同。

Known and unknown samples are “spotted” on to lanes of the TLC plate and migrated with appropriate solvent. The plate is fixed by removing it from the solvent and evaporating solvent from the lanes with the hair dryer. The dissolved compounds will be left behind in the adsorbent material of the lanes. The plate is then developed by spraying it with a solution that induces fluorescence of the compounds present and read under ultraviolet light in a fluorescence analysis cabinet. Unknown compounds are considered to be identical to known standards if they migrate the same distance and exhibit the same degree of fluorescence as standards.

PRIMARY ADVANTAGES OF THIS METHOD: Thin-layer chromatography is a simple, versatile, effective and economical test that is relevant to a wide range of separation problems.

析浦科學(xué)儀器有限公司生產(chǎn)有各種波段的紫外線燈，有手持式多波段紫外線燈、臺(tái)式紫外線燈、紫外線觀察箱等設(shè)備用于TLC薄層色譜分析。