黃色熒光蛋白（Yellow Fluorescent Protein ，YFP）可以看做綠色熒光蛋白的一種突變體，最初來源于維多利亞多管水母（ Aequorea victoria）。相對于綠色熒光蛋白，其熒光向紅色光譜偏移，而這主要是由于蛋白203位蘇氨酸變?yōu)槔野彼?。其更大激發(fā)波長為514 nm，更大發(fā)射波長為527 nm。

常用黃色熒光蛋白： Citrine, Venus, and Ypet。這三種改良的蛋白熒光更亮，更穩(wěn)定，而且成熟更快，因此應(yīng)用廣泛。

上圖：YFP在小鼠上的表達（析浦熒光手電筒GFPfinder-2101觀察效果）

yfp激發(fā)光和發(fā)射光波長

Hyperfolder YFP is a basic (constitutively fluorescent) green/yellow fluorescent protein published in 2022, derived from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with moderate acid sensitivity.

上圖為Hyperfolder YFP的激發(fā)光和發(fā)射光波長光譜圖

Superfolder YFP is a basic (constitutively fluorescent) green/yellow fluorescent protein published in 2005, derived from Aequorea victoria.

mCitrine is a basic (constitutively fluorescent) yellow fluorescent protein published in 2002, derived from Aequorea victoria.

mCitrine YFP的激發(fā)光和發(fā)射光波長光譜圖

mVenus is a basic (constitutively fluorescent) yellow fluorescent protein published in 2006, derived from Aequorea victoria. It is reported to be a rapidly-maturing monomer with moderate acid sensitivity.

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上圖為mVenus的激發(fā)光和發(fā)射光波長光譜圖

YPet is a basic (constitutively fluorescent) yellow fluorescent protein published in 2005, derived from Aequorea victoria. It has moderate acid sensitivity.

上圖為YPet的激發(fā)光和發(fā)射光波長光譜圖

析浦（上海）科學(xué)儀器有限公司生產(chǎn)的便攜式熒光手電筒GFPfinder-2101CY能夠激發(fā)Citrine, Venus, and Ypet等黃色熒光蛋白YFP在植物愈傷、葉片、種子、根系以及各種模式動物上的表達熒光，有手電筒式的熒光手電筒，也有手持式雙波長熒光觀測燈，也有臺式和體視顯微鏡配套使用的熒光適配器。