實(shí)驗室紫外線安全防護(hù)

實(shí)驗室中使用的許多紫外線光源都會發(fā)出高強(qiáng)度的紫外線，從而導(dǎo)致眼睛和皮膚的疼痛燒傷。本培訓(xùn)演示提供了與紫外線暴露相關(guān)的危險以及使用這些來源時應(yīng)采取的安全預(yù)防措施的信息。

上圖：實(shí)驗室常見的紫外線光源警告標(biāo)志

手持式紫外線燈

手持式紫外線燈可用于研究實(shí)驗室，用于凝膠電泳和溴化乙錠染色后的核酸可視化或用于其他目的。

手持式紫外燈操作注意事項

?佩戴紫外線防護(hù)面罩。

?覆蓋所有暴露的皮膚。

?注意可能將紫外線輻射反射到皮膚未受保護(hù)部位的反射表面。

實(shí)驗室人員紫外線防護(hù)裝備

紫外線防護(hù)服：穿全扣實(shí)驗服、長褲和閉趾鞋。確保所有皮膚都得到保護(hù)，包括面部、頸部、手部和手臂。確保你的防護(hù)服沒有縫隙，尤其是手腕和頸部。

紫外線防護(hù)手套：戴上一次性乳膠或丁腈手套，保護(hù)手上裸露的皮膚。不要使用乙烯基手套，因為乙烯基手套會傳播大量光化紫外線。

眼部/面部防護(hù)：始終佩戴全臉防護(hù)紫外線防護(hù)面罩。為了保護(hù)眼睛和面部，請使用印有ANSI Z87.1-1989紫外線認(rèn)證的聚碳酸酯面罩。注意：如果您在有飛濺或拋射危險的情況下工作，您可能還需要在面罩下佩戴安全眼鏡或護(hù)目鏡。

紫外線防護(hù)面罩

只能使用具有紫外線防護(hù)等級的聚碳酸酯面罩。面罩應(yīng)標(biāo)有術(shù)語Z87，以表明面罩符合ANSI標(biāo)準(zhǔn)，至少能提供基本的紫外線防護(hù)。閱讀面罩提供的規(guī)格，以驗證面罩是否提供足夠的紫外線防護(hù)。

紫外線防護(hù)面罩可能無法完全保護(hù)您。例如，假設(shè)你穿著個人防護(hù)裝備在透照器上工作，但你抬頭與附近的人交談。這會使你的喉嚨和下巴暴露在過量的紫外線輻射下。你必須確保沒有裸露的皮膚。切勿為了更仔細(xì)地觀察正在觀察的材料而取下紫外線防護(hù)面罩。

Ultraviolet Light Safety in the Laboratory

Many of the ultraviolet (UV) sources used in the laboratory emit high intensities of UV light, capable of producing painful eye and skin burns. This training presentation provides information about the hazards associated with UV exposure and the safety precautions to take when working with these sources.

Hand Held UV amps

Handheld UV lamps may be used in research laboratories for visualizing nucleic acids following gel electrophoresis and ethidium bromide staining or for other purposes.

Precautions

? Wear a face shield.

? Cover all exposed skin.

? Be aware of reflective surfaces which can reflect UV radiation to unprotected parts of your skin.

Personnel Protective Equipment

Protective Clothing: Wear a fully buttoned lab coat, long pants and closed toe shoes. Make sure that ALL skin is protected, including face, neck, hands and arms. Make sure there are no gaps in your protective clothing, especially at the wrist and neck areas. 

Gloves: Wear disposable latex or nitrile gloves to protect exposed skin on the hands. Do not use vinyl gloves, which can transmit significant amounts of actinic UV. 

Eye/Face Protection: Always wear a full face shield. To protect the eyes and face, use a polycarbonate face shield stamped with the ANSI Z87.1-1989 UV certification. Note: if you’re working with splash or projectile hazards, you may also need to wear safety glasses or goggles under the face shield.

Face Shields

Only use polycarbonate face shields that are rated for UV protection. The face shield should be marked with the term Z87 to indicate that the shield meets the ANSI standard to provide at least basic UV protection. Read the specs provided with the face shield to verify that the shield provides adequate UV protection.

You may not be totally protected by the face shield. For example, suppose that you are working over a transilluminator, wearing PPE, but you are looking up while talking to someone nearby. This would expose your throat and chin to excessive UV radiation. You must be sure that there is no exposed skin. Never remove the face shield in order to get a closer look at the material being visualized.